Stratus fluorometric enzyme immunoassay system evaluated for determination of total thyroxin.
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چکیده
منابع مشابه
"Stratus" fluorometric enzyme immunoassay system evaluated for determination of free thyroxin.
Exceptfor varying the equilibrationtime, the procedurewas essentiallythatof O’Farrell(4) and Laemmli(5) as modifiedbyTracyet al. (5) and Goldsmithet al (7). Protein3 is the extra protein seen when the equilibrationtime is shortened. Proteins I and 2 are the two additionalproteins kientified by Cloixet al (. ProteinsA andBare markerproteinsalsofoundin Cloix’sgel to identifytheSHR proteins.Molecu...
متن کاملEnzyme immunoassay of thyroxin-binding globulin.
We describe a new double-antibody enzyme radioimmunoassay for thyroxin-binding globulin (TBG) in serum. A TBG-beta-D-galactosidase complex was prepared with m-maleimidobenzoyl N-hydroxysuccinimide ester. The mean analytical recovery of TBG added to serum was 99%, sample volume and TBG value were linearly related, and values for TBG determined by this method and those determined by radioimmunoas...
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We describe a double-antibody enzyme immunoassay for free thyroxin (FT4) in serum with use of beta-D-galactosidase conjugated to thyroxin. The method is uninfluenced by thyroxin-binding globulin or albumin. Values for FT4 so determined correlated well with those determined by radioimmunoassay (r = 0.98) and equilibrium dialysis (r = 0.89). The mean variability (CV) within and between assays was...
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This enzyme immunoassay for free thyroxin (FT4) involves simultaneous incubation of sample and thyroxin conjugated to horseradish peroxidase (EC 1.11.1.7) in antibody-coated tubes at room temperature. The test can be performed in 90 min. It is not influenced by variations in concentrations of thyroxin-binding globulin (TBG), thyroxin-binding prealbumin, or albumin. With increased concentrations...
متن کاملThe Anagen system for automated fluorometric immunoassay.
We describe a new discrete microprocessor-controlled analyzer, the AN2000, which fully automates fluorometric immunoassays by using a magnetic separation of the solid phase and an alkaline phosphatase label. It can operate in random-access or batch mode with a dwell time typically less than 20 min. The analysis rate is 75 samples per hour and the system can hold refrigerated reagents for as man...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1988
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/34.2.433